Journal: Frontiers in Nutrition

Article Title: Bifidobacterium adolescentis CCFM1447 effectively alleviates osteoporosis by enriching intestinal flora capable of vitamin D conversion

doi: 10.3389/fnut.2025.1647671

Figure Lengend Snippet: Effects on serum (A) 25(OH)D, (B) 1,25(OH) 2 D, (C) ALP, (D) Ca + , (E) OCN, (F) PINP concentration in mice. * indicates significant differences from the model group: * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Osteocalcin (OCN) and Procollagen type I N-propeptide (PINP) levels were measured using commercial Mouse ELISA kits (E-EL-M0864, E-EL-M0233, Elabscience Biotechnology).

Techniques: Concentration Assay

Journal: Frontiers in Nutrition

Article Title: Bifidobacterium adolescentis CCFM1447 effectively alleviates osteoporosis by enriching intestinal flora capable of vitamin D conversion

doi: 10.3389/fnut.2025.1647671

Figure Lengend Snippet: (A) Levels of 1,25(OH) 2 D in the supernatants of key differential bacteria after separate cultures; Effects of key differential flora on serum (B) 25(OH)D, (C) 1,25(OH) 2 D, (D) OCN, (E) PINP concentration in mice. * indicates significant differences from the model group: * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Osteocalcin (OCN) and Procollagen type I N-propeptide (PINP) levels were measured using commercial Mouse ELISA kits (E-EL-M0864, E-EL-M0233, Elabscience Biotechnology).

Techniques: Bacteria, Concentration Assay

Journal: Antioxidants

Article Title: Protective Effects of Melon Extracts on Bone Strength, Mineralization, and Metabolism in Rats with Ovariectomy-Induced Osteoporosis

doi: 10.3390/antiox8080306

Figure Lengend Snippet: ELISA analysis of bone markers. ( a ) Alkaline phosphatase (ALP), ( b ) osteocalcin, ( c ) C-telopeptide cross-linked collagen type I (CTXI), and ( d ) calcium. * p < 0.05.

Article Snippet: The level of C-telopeptide cross-linked collagen type I (CTXI) as a bone resorption marker was analyzed using a rat CTXI ELISA kit (Elabscience Biotechnology Inc., Houston, TX, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of translational medicine

Article Title: Lumican is elevated in the lung in human and experimental acute respiratory distress syndrome and promotes early fibrotic responses to lung injury.

doi: 10.1186/s12967-022-03597-z

Figure Lengend Snippet: Fig. 1 Lumican, fibronectin, COL1A1, and COL3A1 levels and their correlations in human subjects. Serum and BALF lumican levels (A, B) in the ARDS group and the control groups (spontaneously breathing controls and ventilated controls). BALF levels of fibronectin (C), COL1A1 (D), and COL3A1 (E) were measured in all groups. BALF lumican levels were positively correlated with (F) BALF fibronectin levels, (G) BALF COL1A1 levels, and (H) BALF COL3A1 levels. Values are expressed as medians with interquartile ranges. The significance of between-group differences was examined using Kruskal–Wallis test. p < 0.05 was considered statistically significant. Correlations were investigated using Spearman’s correlation analysis (r indicates the correlation coefficient). ARDS acute respiratory distress syndrome, BALF bronchoalveolar lavage fluid, COL1A1 alpha-1 type I collagen, COL3A1 alpha-1 type III collagen

Article Snippet: The concentration of lumican in the cell culture supernatants and human BALF alpha-1 type I collagen (COL1A1) levels were measured using ELISA kit (R&D Systems) according to the manufacturer’s instructions.

Techniques: Control

Journal: Journal of Bone and Mineral Research

Article Title: New lens on congenital mild bone fragility: a novel Col1a1 knockout mouse model for osteogenesis imperfecta type 1

doi: 10.1093/jbmr/zjaf138

Figure Lengend Snippet: Altered collagen gene expression and serum biomarkers in hiOI mice. (A) Serum P1NP levels were significantly reduced in hiOI mice at 8 and 24 wk of age. (B) Serum TRAcP 5b levels remained normal in hiOI mice. (C-D) Real-time quantitative PCR analysis of Col1a1 and Col1a2 mRNA expression and their ratio in 20 hiOI and 20 WT mice at 8 wk (C) and 24 wk of age (D). (E) Serum P1NP levels were positively correlated with the Col1a1/Col1a2 mRNA expression ratio in bone at 8 wk ( p = .0088, r = 0.4137) and 24 wk ( p < .0001, r = 0.6246). (F) The Col1a1/Col1a2 ratio was correlated with trabecular and cortical bone volume in 8-wk-old mice but not in 24-wk-old mice. p -values are indicated as follows: ≤.05 ( * ), ≤.01 ( ** ), ≤.001 ( *** ), ≤.0001 ( **** ). Data shown as mean ± SD. All comparisons between WT and hiOI data were performed using an unpaired t -test. Gender subgroups were analyzed using a two-way ANOVA with a main effect and multiple comparisons. Correlations between P1NP levels, collagen mRNA expression ratios, and bone volumes were assessed using Pearson correlation analysis.

Article Snippet: The serum levels of procollagen type I N-terminal propeptide (P1NP) (P1NP EIA kit, AC-33F1, Immunodiagnostic systems), a bone formation marker, were assessed in 8-wk-old and 24-wk-old mice by ELISA according to the manufacturer’s protocol.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing

Journal: Journal of Bone and Mineral Research

Article Title: New lens on congenital mild bone fragility: a novel Col1a1 knockout mouse model for osteogenesis imperfecta type 1

doi: 10.1093/jbmr/zjaf138

Figure Lengend Snippet: Reduced bone collagen expression in hiOI mice. (A) Western blot analysis of α1(I) and α2(I) collagen protein bands extracted from ulnae bones of WT ( n = 3) and hiOI ( n = 3) mice. (B) Quantification showing the percentage reduction of α1(I), α2(I), total collagen type I, and the Col1a1 / Col1a2 protein ratio in hiOI relative to WT ulnae. (C) Genotype, Col1a1 / Col1a2 mRNA ratio, serum P1NP levels, and total collagen type I (normalized to tissue weight) in ulnae. (D) A positive correlation between serum P1NP levels and total collagen type I measured by western blot is shown. Statistical significance indicated by * for p ≤ .05.

Article Snippet: The serum levels of procollagen type I N-terminal propeptide (P1NP) (P1NP EIA kit, AC-33F1, Immunodiagnostic systems), a bone formation marker, were assessed in 8-wk-old and 24-wk-old mice by ELISA according to the manufacturer’s protocol.

Techniques: Expressing, Western Blot